Extended Data Fig. 9: Dual mode interactions between ribosomes and piRNA precursors at uORFs and UDRs.
From: Ribosomes guide pachytene piRNA formation on long intergenic piRNA precursors
a, Schematic of affinity purification procedure for ribosome-associated RNAs. Renilla RNA was used as a negative control. Firefly RNA was used as a spike-in for normalization. b, RNA enrichment in affinity-purified ribosomes versus control monosome IP from littermates (without Cre). The abundance of transcripts was quantified using qRT-PCR with three independent biological samples (mean ± standard deviation; n = 3 independent experiments). *p < 0.05 by one-side Student’s t-test comparing the RNAs in vivo with the in vitro-added negative control. Each data point was overlaid as dot plots. c, Histology of testis sections with (right) and without (left) puromycin treatment harvested at different time points. Experiments have been repeated for two times independently with similar results. Puromycin did not disrupt seminiferous tubule structures or lead to visible morphological changes up to 8 hours after intra-testicular injection. Scale bar, 50 μm. d, Scatterplot of mRNA abundance between untreated and puromycin treated adult mouse testis. Each data point represents an mRNA. Sample size n = 8,623 mRNAs. Tpm, transcripts per million. Pearson’s correlation and the corresponding p-value based on two-sided Pearson’s correlation test is listed. Statistical Source Data are provided in Source Data Extended Data Fig. 9.
