Extended Data Fig. 9: Tagged HSF1 FRAP traces and statistics.
From: HSF1 phase transition mediates stress adaptation and cell fate decisions
a–d, Fluorescent recovery after photobleaching (FRAP) single-cell traces and exponential fits. Region of interest photobleached after 5 seconds from start of imaging. a,b, Whole focus FRAP traces after 2.5 µM MG132 treatment a, 2 hours after treatment and b, 8 hours after treatment. c,d, Half-focus FRAP traces after 2.5 µM MG132 treatment. c, 1.5 hours after treatment. d, 8 hours after treatment. e, Whole focus FRAP Thalf recovery time estimates after 2.5 µM MG132 addition (mean +/- SEM, n = 28, 17, 27, 19, 19, 10 foci per time point). f,g, Whole focus FRAP on HCT15 HSF1-YFP parameter estimates 1.5 and 5 hours after 5 µM MG132 treatment. f, Immobile fraction and g, Thalf recovery time (n = 14 foci, two-sided KS p-value). h, Half-focus FRAP Thalf recovery time estimates 1.5 and 8 hours after the addition of 2.5 µM MG132 (mean +/- SEM, n = 30 and 32 foci per time point, two-sided KS p-value). Source Data are provided in Source Data Extended Data Fig. 9.
