Extended Data Fig. 1: HSF1 foci quantification in colon adenocarcinoma tissues.

a,b, Composite images of immunofluorescence data of HSF1 (clone 10H8, green) and DAPI nuclear stain (blue) from a tissue sections of colon adenocarcinoma with adjacent non-neoplastic colon tissue. T, tumor; N, normal. a, Insets are two regions at the borders between tumor and normal tissue. b, Insets 1-3 tumor tissue, inset 4 normal colon tissue. c, High power composite image of t-CyCIF data showing CDX2 (red), αSMA (purple) CD45 (yellow). d, Quantification of t-CyCIF data from three colon adenocarcinoma whole tissue sections indicating the lineage of cells positive for HSF1 foci (“Foci+”, HSF1-FI > 0.05). e, Bar graph of Foci+ cells in colon adenocarcinoma cases (n = 93 patients; from tissue microarray (TMA); four cores per patient, average +/- SEM) from t-CyCIF data. f, Scatter plot of the frequency of Foci+ cells in tumor versus non-tumor compartments (CD45+ immune cells, yellow; αSMA+ stromal cells, purple). Each dot represents CD45 or αSMA data from a single core from TMA. g, Bar graph of Foci+ cells from t-CyCIF data from TMA (tumor, red; immune, yellow; stroma, purple, n = 87 patients, average + SEM). h, 2D single-cell, spatially-averaged heatmap of t-CyCIF data of HSP70 and HSP90 expression normalized over median expression (scalebar 1mm). i, Kernel density (KD) estimated frequency distribution plot of HSP70 chaperone protein expression in normal colon tissue (red) and adenocarcinoma tissue (blue) from t-CyCIF. j, Top, 2D density plot of HSF1 concentration versus chaperone proteins from t-CyCIF of colon adenocarcinoma in Fig. 1b, c (n ~ 250,000 cells). Bottom, corresponding scatter plot (subsampling of 1,000 cells, one dot per cell; dashed line, linear fit). k, Scatter plot of mean HSF1-FI versus mean chaperone levels of the TMA (one dot per core; color represents the number of cells in core). Source data are provided in Source Data Extended Data Fig. 1.

Source data