Extended Data Fig. 2: HSF1 foci quantification in myeloma/plasmacytoma tissues.
From: HSF1 phase transition mediates stress adaptation and cell fate decisions
a,b, 2D cell density plots of two antibodies against Hsp70 (a) and Hsp90 (b) protein from 9 patient samples of plasmacytoma/myeloma from Fig. 1e–k. c–f, Statistics for each of the 9 plasmacytoma/myeloma patient samples. c, Single-cell scatter plot of signal from Hsp70 EPR16892 antibody versus Hsp90 C45G5 antibody. Red lines represent the threshold identified in Fig. 1 for which cells were considered positive or negative for the corresponding chaperone protein expression. d, percentage of cells with HSF1 foci (HSF1-FI > 0.05) divided by cells positive or negative for both HSP70 and HSP90 chaperones (“Chap++” and “Chap–” respectively). e, Number of cells in each Chap++ and Chap–category. f, Mean HSF1 levels per chaperone category. Source data are provided in Source Data Extended Data Fig. 2.
