Extended Data Fig. 5: Relationship between HSF1 foci and cell death by time-lapse microscopy.
From: HSF1 phase transition mediates stress adaptation and cell fate decisions
a, Time-lapse microscopy traces of HSF1-GFP cell concentration from single cells followed for 12 hours at 30 minute intervals post 2.5 µM MG132 (mean +/- SEM). Cells are separated between those that survived (blue, n = 96 cells) and those that died (red, n = 54 cells) as in Fig. 3a. b,c, Bar graph of HSF1-FI (b) and HSF1 concentration (c) at 5 and 10 hours after 2.5 µM MG132 comparing cells that survive (blue) and cells that die (red) during the time lapse microscopy experiment in panel a, and Fig. 3a (two-sided KS p-value with 0.05 significance threshold, mean +/- SEM). d, Immunofluorescence on U2OS cells with mVenus protein endogenously inserted in C-terminus of HSF1 genomic locus (HSF1-mVenusKI). Two independent clones (called “B12” and “C5”) were analysed. Scalebar = 10 µm. e, Quantification of time-lapse microscopy on U2OS HSF1-mVenusKI clone B12 and C5 after 5 µM MG132. Red = cells that died during the 14 hours experiment (n = 153 cells and n = 156 cells), green = cells that survived (n = 101 cells and n = 199 cells, mean +/- SEM). Source Data are provided in Source Data Extended Data Fig. 5.
