Extended Data Fig. 8: Heat shock does not induce a universal protein dephosphorylation and kinase degradation.
From: Heat stress activates YAP/TAZ to induce the heat shock transcriptome
a-b, High density HEK293A cells were heat shocked at 43oC for the indicated times and cell lysates were collected for Western blot to detect the phosphorylation and protein levels of the indicated proteins. c, HEK293A cells were transiently transfected with GST-MST1. 24 h after transfection, cells were subjected to heat shock for the indicated times. Glutathione Sepharose 4B beads were used to purify GST-MST1. Phosphorylation of GST-MST1 was analyzed by Western blot with pMST1 (Thr183) antibody. Immunoblotting for a-c has been performed two times with similar results. Source data are available online.
