Extended Data Fig. 7: Characterizing the expression profile and effect of ssrp1a on HSPCs.
a–f, WISH of embryos injected with ssrp1a-MO and probed with efnb2a (a,b), gata1 (c,d), and cdh5 (e,f) at 26 hpf. No effect is seen on dorsal aorta, red blood cell, or vasculature formation upon ssrp1a knockdown. g-o, WISH of supt16h, ssrp1a, and ssrp1b for WT embryos at 14-16 hpf with a dorsal view (g-i), and 24 hpf (j-l) and 32 hpf with a posterior lateral view (m-o). p-w, TUNEL of uninjected (p-s) and ssrp1a-MO (t-w) embryos at 32 hpf. Confocal images of TUNEL and DAPI (left) and TUNEL only (right) are shown. x, Quantification of the number of apoptotic cells in ssrp1a morphants from (p-w) based on TUNEL for the trunk region (Represented as mean ± s.d., one-way ANOVA with post-hoc Tukey, n = 10, P < 0.0001, N.S. = not significant). y, Mean fluorescence level of Tp1:GFP of the DA calculated in ImageJ based on Integrated Density (Represented as mean ± s.d., two- tailed t-test, nWT = 11, nMO = 15, *P = 0.0147, N.S. = not significant). z,a’, WISH of notch1b in WT sibling and ssrp1a-/- embryos at 28 hpf. Data representative of 2 (g-o) biological replicates. Bar, 100 μm. Source data provided in Supplementary Table 5.
