Extended Data Fig. 1: Characterizing the causal mutation from our forward genetic screen.
a, Diagram of the forward genetic screen strategy. b,c, Mapping of RNA-seq using RNAmapper with whole genome view (b) and specifically looking at the linked interval on Chr 7 (c). d, Position and RNA-seq coverage of SNP on supt16h resulting in a premature stop codon. e,f, Expression of supt16h-/- based on RNA-seq (e) (Represented as mean ± s.d., two-tailed Student’s t-test, n = 3, P = 0.0005) and RT-qPCR (f) (Represented as mean ± s.d., two-tailed Student’s t-test, n = 3, P = 0.0024) for cmyb. For RT-qPCR, expressions are relative to WT sibling. g-j, WISH of WT embryos injected with supt16h-MO for runx1 (blue arrowheads) at 28 hpf and cmyb at 36 hpf. k,l, Representative confocal of Tg(cmyb:GFP;kdrl:mCherry) embryos injected with supt16h-MO from one independent experiment. Double positive HSPCs indicated by white arrowheads at 48 hpf. DA = dorsal aorta; V = vein. m, Quantification of double positive HSPCs from (g and h) (Represented as mean ± s.e.m., two- tailed t-test, n = 10, P < 0.0001). Bar, 100 μm. Source data provided in Supplementary Table 5.
