Extended Data Fig. 3: Scattering of cells during single-cell integration and gene regulatory networks analysis, related to Fig. 1.
From: Evaluating totipotency using criteria of increasing stringency
a, Cells are binned according to their position in the integration UMAP in Fig. 1e depending on whether they occupy a position defined as ICM, TE, PE, EPI E4.5, EPI E5.5 or EPI E6.75. ScRNAseq profiles of L-EPSCs generated in this study are merged with published EPSCs signatures from the Liu lab (Liu EPSCs)35. Modular scores are then presented for each subcluster of each stem cell type. The modular scores are based on the top100 differentially expressed gene signatures defined by the corresponding embryo data. The number of cells in each group can be found in Source data Extended Fig. 3. b, UMAP constructed based on the activity of gene regulatory networks in 2iLif ESCs, embryonic stages E3.5 ICM, E4.5 EPI, E5.5 EPI, L-EPSCs and D-EPSCs.
