Extended Data Fig. 6: Ectocervical stemness and differentiation.
From: Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia
a, Brightfield images of human organoids at P1 or P8 from 2D-ectocervical cells and long-term passage (P5) of organoids in 3D. Percentages indicate organoid-forming efficiency. b, Percentage of Ki67+ proliferating cells in EO-ecto and DO-ecto organoid-derived cells. c, Heatmap of genes concordantly up- or downregulated in ectocervical stem cells vs differentiated cells and similar comparisons to the ground state stem cell dataset (see Methods for details). Expression levels in stem cells and their corresponding differentiated cells from 13 different tissue types. DA-Distal Airway; NT-Nasotubular epithelium; TB-Tracheobronchial epithelium; FT-Fallopian tube; CA-Colon ascendens, CD-Colon descendens, CT-Colon transversum, DD-Duodenum, ES-Esophagus, IL-Ileum, JJ-Jejunum, K5-Keratin 5+ oesophagal cells, K7-Keratin 7+ oesophagal cells. d, Phase-contrast images of human ectocervical organoids in the presence or absence of DBZ. Data representative of n = biologically independent experiments from 3 donors.
