Fig. 8: Molecular expression patterns of lineage markers in healthy tissue, organoids and cervical cancers.
From: Opposing Wnt signals regulate cervical squamocolumnar homeostasis and emergence of metaplasia
a,b, Left: tiled images of cervix tissue sections from human (a) and mouse (b), including stratified and columnar epithelium and the transition zone, immunolabelled for KRT5 and KRT7; nuclei in blue. Boxed areas are magnified on the right. Images are representative of biologically independent experiments from three mice or human samples. c, Confocal images of human ectocervical stratified and endocervical columnar organoids immunolabelled for KRT5 and KRT7; nuclei in blue. Images representative of n = 3 donors. d, Labelling for bimodally-expressed proteins in the normal cervix, SCC and ADC. Tissue sections from normal tissue, SCC and ADC of the cervix were stained with haematoxylin and eosin or labelled with antibodies against KRT5, KRT7, KRT8, AGR2, GDA, MUC5B and CSTA; nuclei in blue. Magnifications of the boxed areas are shown in insets. Data representative of biologically independent experiments from five humans. e, Model depicting the two epithelial lineages and Wnt–Notch microenvironment in the transition zone and during squamous metaplasia.
