Extended Data Fig. 8: Translation fidelity is unaffected in RPS23R67K/+.
From: Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition
a, The stop codon readthrough reporter comprises 10X Upstream Activator Sequences (UAS), which confers GAL4 responsiveness, the 5’ UTR from Syn21, the coding region of Firefly luciferase (Fluc), a STOP codon (UGAC), a flexible linker, the coding region of Nanoluciferase (Nluc), and the 3’UTR from p10. b, Quantification of the Nluc/Fluc ratio, measured from whole larvae lysates and normalised to that in control larvae. Statistical analysis: 4 replicates for each condition. Error bars denote standard deviation. A two-tailed unpaired t-test was carried out. P>0.05, no significant increase was seen in RPS23R67K/+ larvae. Genotypes for each figure panel are available in Supplementary Table 1. Source data is available for this figure.
