Extended Data Fig. 10: Effect of proteasome inhibition on the rate of apoptosis in RP-deficient tissues.
From: Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition
a, Extent of apoptosis (coverage of Dcp1 immuno-reactivity) in the pouch of discs of genotypes indicated. Rpt6RNAi denotes rotund-gal4-driven expression of a Rpt6RNAi transgene.. This particular Rpt6RNAi transgene had only a minor effect on apoptosis in wildtype tissue. Expression of this RNAi transgene did not enhance the rate of apoptosis in RPS23R67K heterozygotes. b, The effect of stronger proteasome knockdown (with Rpn2RNAi) on the rate of apoptosis in RP-deficient tissue could not be assessed because it triggered extensive apoptosis in otherwise wild type imaginal discs. Statistics: error bars denote standard deviation. n = 9 discs for each genotype. A two-tailed unpaired t-test was carried out. P > 0.05, no significant difference was seen. Genotypes for each figure panel are available in Supplementary Table 1. Source data is available for this figure.
