Extended Data Fig. 1: Protein synthesis and its regulation in Rps3+/− cells.
From: Proteotoxic stress is a driver of the loser status and cell competition
(a-c) AHA (grey) protein synthesis assay in wing discs harboring either Rps3+/− clones (GFP-positive) (a) or clones overexpressing 4EBPTA (GFP-positive) (b), and corresponding quantification (n = 7 and 7, respectively, two-sided paired t-test without p-adjustment for multiple comparisons) (c). (d-e) OPP (green) protein synthesis assay in a wing disc expressing mahj-RNAi in the P compartment (positively labelled with RFP) (d) and corresponding quantification (n = 10, two-sided Wilcoxon signed-rank test) (e). (f-g) An RpS3+/− wing disc expressing GADD34 in the P compartment and labelled with phospho-eIF2α (red) (f), and corresponding quantification (n = 10, two-sided paired t-test) (g). (h-i) GST-GFP reporter (green) activation in an RpS3+/− wing disc expressing GADD34 in the P compartment (h), and corresponding quantification (n = 10, two-sided paired t-test) (i). For all micrographs, scale bars correspond to 50 µm. For all quantifications provided, the horizontal line represents the mean and whiskers indicate 95% confidence intervals. All n numbers refer to the number of individual wing discs.
