Extended Data Fig. 2: The role of autophagy in prospective losers.
From: Proteotoxic stress is a driver of the loser status and cell competition
(a) GstD1-GFP signal (green) in a RpS3+/− wing disc expressing Puc in P cells (labelled by the absence of Ci, magenta). (b-c) Apoptotic cell death, as detected by anti-cleaved Caspase-3 reactivity (red), in wing discs of an atg8+/− heterozygote (b, left), RpS3+/− heterozygote (b, middle), or atg8+/−, RpS3+/− transheterozygote (b, right) and corresponding quantification (n = 9, 8, and 9 respectively, two-sided two sample Kolmgorov-Smirnov test without p-adjustment for multiple comparisons) (c). (d) Apoptotic cell death, as detected by anti-cleaved Caspase-3 reactivity (red), in wing discs of an atg13+/− heterozygote (d, left), RpS3+/− heterozygote (d, middle), or atg13+/−, RpS3+/− transheterozygote (d, right). (e-f) Apoptotic cell death, as detected by anti-cleaved dcp1 antibody staining (red), in wing discs of a p62+/− heterozygote (f, left), RpL27A+/− heterozygote (f, middle), or RpL27A+/−, p62+/− transheterozygote (f, right) and corresponding quantification (n = 10, 10, and 12 respectively, two-sided Mann-Whitney U test without p-adjustment for multiple comparisons) (e). (g-i) Wing discs harboring RpS3+/− clones (GFP-positive) (h, left), RpS3+/− clones expressing atg1-RNAi (GFP-positive) (h, middle), or RpS3+/− clones expressing atg9-RNAi (GFP-positive) (h, right) stained with cleaved-dcp1 (red) and corresponding quantification of border cell death (n = 16, 12, and 9 respectively, two-sided Mann-Whitney U test without p-adjustment for multiple comparisons) (g) and clone coverage (n = 16, 12, and 9 respectively, two-sided student’s t-test without p-adjustment for multiple comparisons) (i). For all micrographs, scale bars correspond to 50 µm. For all quantifications provided, the horizontal line represents the mean and whiskers indicate 95% confidence intervals. All n numbers refer to the number of individual wing discs.
