Extended Data Fig. 9: Generation and characterization of RaptorD/D knock-in mice.
From: LATS suppresses mTORC1 activity to directly coordinate Hippo and mTORC1 pathways in growth control
a, sgRNA sequence and part of ssODN sequence used for generating RaptorD/D mice. b, Analysis of mouse genomic DNA by SfcI digestion. c, Sanger sequencing results of RaptorD/D mouse genomic DNA. d. Frequency of genotypes produced from Raptor+/D mouse intercrosses. e, Representative images of 2-week-old and 8 week-old mice. f, Body weight of mice. Data were shown as mean ± s.e.m. (n = 44 mice/group). g, Growth of female and male mice. Data were shown as mean ± s.e.m. (female, n = 20 mice/group; male, n = 18 mice/group). h, Body weight of mice fed with high fat diet (HFD). Data were shown as mean ± s.e.m. (n = 7 mice/group). i, Representative images of Raptor+/+ and RaptorD/D mice fed with HFD for 11 weeks. j, Weight of white adipose tissue (WAT), brown adipose tissue (BAT) and liver was shown as percentage of body weight in mice that were on an HFD for 11 weeks. Data were shown as mean ± s.e.m. (n = 7/group). k, Representative image of bodipy staining of liver sections from Raptor+/+ and RaptorD/D mice that were on an HFD for 11 weeks. l, Organ sections were analysed by immunohistochemistry for pS6 (pS235/S236) levels. Scale bars, 50 μm for spleen and 100 μm for brain. m, IB analysis of WCLs derived from indicated organs. n, IB analysis of WCLs derived from livers and hearts of mice depleted Lats1/2. o, AAV-mediated knockdown of Lats1/2 increases liver size. Data were shown as mean ± s.e.m. (n = 5 mice/group). P values in f, g, j and o were calculated using two-tailed Student’s t-test. The experiments in b, l, m and n were repeated n = 2 independent experiments, with similar results obtained. Unprocessed immunoblots are shown in Source Data Extended Data Fig. 9. Statistical source data are available in Statistical Source Data Extended Data Fig. 9.
