Extended Data Fig. 10: SFRP2 regulates survival of disseminated indolent breast cancer cells.
From: Crosstalk with lung epithelial cells regulates Sfrp2-mediated latency in breast cancer dissemination
a, Quantification of metastatic burden two weeks after intravenous injection of human indolent breast cancer cell. n = 6 slices across 3 mice for T47D-DBM cells and n = 10 slices across 3 mice for MCF7 cells. Plots show data as whisker plots: midline, median; box, 25–75th percentile; whisker, minimum to maximum. Unpaired t-test. b, In vitro growth curves of Control and SFRP2 over-expressing D2.0R and 4T07 cells. Confluency values at indicated time points were log10-transformed and linear regression was calculated. Line was forced to go through the origin. Solid line, mean of best-fit line; dashed lines, 95% confidence bands. n = 2 independent experiments. c, Proximity of disseminated SFRP2-overexpressing D2.0R cells to indicated lung stromal cells at 3 or 14-days post-injection. Lung slices from 3 mice injected with D2.0R-EGFP cells have been stained with multiple markers for different stromal subpopulations. Graphs indicate the percentage of EGFP + cells in contact with each stromal cells subtype (black: in contact; white: not in contact). Staining as in Extended Data Fig. 1f. d, Schematic illustration of the signaling between AT1 cells and breast cancer cells that supports metastatic persistence.
