Extended Data Fig. 2: Lung stromal cells regulate proliferation of breast cancer cells in vitro.
From: Crosstalk with lung epithelial cells regulates Sfrp2-mediated latency in breast cancer dissemination
a, Relative mRNA levels of stromal cell-type specific markers of the different cellular populations included in the lung coculture system. AT1, alveolar type1 cells; AT2, alveolar type2 cells. Dots are means from independent experiments (n = 3). Unpaired t-test. b, Percentage of Ki67 + -D2.0R-EGFP or -D2A1-EGFP cells cultivated together with lung stromal cell lines in Mitogen Low-Nutrients Low medium (MLNL) for 4 days. Mean normalized pooled samples (n = 7) from independent experiments (n = 2). Data presented as whisker plot: midline, median; box, 25–75th percentile; whisker, minimum to maximum. Unpaired t-test. c, Growth curves of D2.0R-EGFP and D2A1-EGFP cells in vitro with permissive (MHNH) or restrictive (MLNL) medium. d, D2.0R-EGFP cells were cocultured with lung stromal cells for 5 days (or on air-permeable surface only as control), isolated by fluorescence-activated cell sorting (FACS), and their growth kinetic in vitro in MLNL on standard plastic plates measured over time (lines are overlapped). n = 2 independent experiments. For c and d, Confluency values at indicated time points were log10-transformed and linear regression was calculated. Line was forced to go through the origin. n = 6 biological replicates/group, n = 2 independent experiments. Solid line, mean of best-fit line; dashed lines, 95% confidence bands.
