Extended Data Fig. 4: Mass cytometry analysis reveals signaling pathways involved in the crosstalk between AT1 and indolent breast cancer cells.
From: Crosstalk with lung epithelial cells regulates Sfrp2-mediated latency in breast cancer dissemination
a, Schematics representation of the experimental outline of mass cytometry assay. b, Heatmaps of EMD values showing the activation of relevant markers in AT1-like cells cocultured with D2.0R or MCF7. n = 1 experiment. c, Plot showing increase phospho-HistoneH3 (S28) signal in AT1-like cells co-cultured with D2.0R cells. Representative of two independent experiments. d, DREVI plots showing the relationship between the indicated phospho-antibody signals in D2.0R monocultures or cocultures with AT1-like cells (DREMI score in upper left corner). Representative of two independent experiments. e, Number of cells after the indicated treatment (for two days) relative to untreated cells. Mean values from n = 3 (for Control, EGFRi, SFKi) and 4 (for MEKi) independent experiments. One-way ANOVA test. f, Histogram of EMD values showing the inhibition of P-ERK abundance in D2.0R cells cocultured with AT1-like cells. Bars show the average of two technical replicates. g, Plot shows the area of D2.0R colonies ten days after intravenous delivery into either control Balb/C nude mice (n = 1147 across 5 mice) or Trametinib treated mice (n = 179 across 4 mice, MEKi). Mann-Whitney test. e, g plots show data as whisker plots: midline, median; box, 25–75th percentile; whisker, minimum to maximum.
