Extended Data Fig. 4: The effect of Yaf2 on propagation of H2AK119ub1.

a, Heat map analysis of H2AK119ub1 across H2AK119ub1 enriched peaks (±3 kb) in wild type, Rybp^-/-, Yaf2^-/- and Rybp^-/-/Yaf2^-/- mESCs, ranked from highest to lowest ChIP-seq signal of H2AK119ub1 in wild type mESCs. b, ChIP-seq cumulative enrichment deposition centered at peak summit of H2AK119ub1 in wild type, Rybp^-/-, Yaf2^-/- and Rybp^-/-/Yaf2^-/- mESCs. The ChIP-seq assays in a, b have been performed in duplicatesusing two biologically independent samples (once per sample). c, ChIP-qPCR analysis of H2AK119ub1 at selected gene loci in wild type, Rybp^-/-, Yaf2^-/- and Rybp^-/-/Yaf2^-/- mESCs (n=3 biologically independent samples). Data represents mean ± s.d. IgG served as ChIP negative control. ChIP enrichments are normalized to input. d, Representative immunoblot of YAF2, RING1B, H2AK119ub1, RYBP and H3 in wild type, Rybp^-/-, Yaf2^-/- and Rybp^-/-/Yaf2^-/- mESCs out of 3 independent experiments. e, Representative quantitative-immunoblot of H2AK119ub1 in Rybp^-/-/Yaf2^-/-, Rybp^-/- and Yaf2^-/- mESCs out of 3 independent experiments. f, Quantitative signals of H2AK119ub1 immunoblot by Image J, normalized to H3 signal (n=4 biologically independent experiments). Data represents mean ± s.d. Unprocessed blots and statistical source data are available in Source Data.

Source data