Extended Data Fig. 6: Inheritance of H2AK119ub1 after washing out target signal by Dox.
a, ChIP-qPCR analysis of HA (left) and H2AK119ub1 (right) across the 8xtetO integrating locus in TetR-tetO targeting mESCs, either treated by Dox 36 hrs or untreated. Dox was added 24 hrs after transient transfection of RING1B-TetR-HA (n=3 biologically independent samples). Data represents mean ± s.d. ChIP enrichments are normalized to input. b, ChIP-qPCR analysis of H2AK119ub1 across the 8xtetO integrating locus in TetR-tetO targeting mESCs with RYBP-TetR-HA stable expression, either treated by dox 24 hrs, 48 hrs and 72 hrs or untreated (n=3 biologically independent samples). Data represents mean ± s.d. ChIP enrichments are normalized to input. c, ChIP-qPCR analysis of H2AK119ub1 across the 8xtetO integrating locus in TetR-tetO targeting mESCs, either treated by Dox 36 hrs or untreated (n=3 biologically independent samples). Data represents mean ± s.d. ChIP enrichments are normalized to input. d, ChIP-qPCR analysis of H2AK119ub1 across the 8xtetO integrating locus in TetR-tetO targeting mESCs with RYBP-TetR-HA or RYBPTF/AA-TetR-HA stable expression, either treated by Dox 36 hrs or untreated, IgG served as ChIP negative controls (n=3 biologically independent samples). Data represents mean ± s.d. ChIP enrichments are normalized to input. Statistical source data are available in Source Data.
