Extended Data Fig. 9: Overlapping roles of RYBP-PRC1 and histone H1.
a, A scatter plot showing the fold changes of H2AK119ub1 peaks in RybpTF/AA and H1-TKO mESCs, both relative to wild type mESCs. Red dots represent peaks with H2AK119ub1 reduction > 1.5-fold. b, Venn diagrams depicting the overlap genes identified as showing a greater than 2-fold expression change both in RybpTF/AA and H1-TKO mESCs, relative to wild type mESCs (n=3 biologically independent samples). Gene numbers with expression changes were shown, respectively. c, Representative immunofluorescence images of NANOG and TUBB3 during NPC differentiation in wild type, RybpTF/AA and H1-TKO mESCs out of 3 independent experiments. DNA was stained by DAPI. Scale bars are 50μm. d, RT-qPCR analysis of expression of selected genes during mNPCs differentiation in wild type, RybpKO, RybpTF/AA and H1-TKO mESCs, normalized with reference control of GAPDH and to wild-type mESCs (n=3 biologically independent samples). Data represents mean ± s.d. e, Venn diagrams depicting the overlap genes identified as showing a greater than 2-fold expression change both in RybpTF/AA and H1-TKO mNPCs, relative to wild type mNPCs (n=3 biologically independent samples). Gene numbers with expression changes were shown, respectively. Statistical source data are available in Source Data.
