Extended Data Fig. 2: Changes of RING1B occupancy in Rybp-/-, Eed-/- and Eed-/-/Rybp-/- mESCs.
a, A Venn diagram showing the overlap of H2AK119ub1 (orange) and RYBP (purple) peaks in mESCs. Numbers represent the total peaks of H2AK119ub1 (orange) and RYBP (purple), respectively. b, Representative immunoblot of RING1B, CBX7, EZH2 and H2AK119ub1 in wild type and Rybp knockout cells out of 3 independent experiments. c, A scatter plot shows changes of RING1B reads density upon RYBP deletion in mESCs. d, ChIP-qPCR analysis for RING1B at selected gene loci in wild type, Rybp knockout and RybpTF/AA mESCs, IgG as negative control (n=3 biologically independent samples). Data represents mean ± s.d. ChIP enrichments are normalized to input. e, Heat map analysis of RING1B across RING1B enriched peaks (±3 kb) in wild type, Rybp-/-, Eed-/- and Eed-/-/Rybp-/- mESCs, ranked from highest to lowest ChIP-seq signal of RING1B in wild type mESCs. f, ChIP-seq cumulative enrichment deposition centered at peak summit of RING1B in wild type, Rybp-/-, Eed-/- and Eed-/-/Rybp-/- mESCs. The ChIP-seq assays in a, c, e, f have been performed in duplicates using two biologically independent samples (once per sample). g, ChIP-qPCR analysis of RING1B at selected gene loci in wild type, Rybp-/-, Eed-/- and Eed-/-/Rybp-/- mESCs, IgG served as ChIP negative control (n=3 biologically independent samples). Data represents mean ± s.d. ChIP enrichments are normalized to input. Unprocessed blots and statistical source data are available in Source Data.
