Extended Data Fig. 3: The CC and WW domains are required for TAZ to form nuclear puncta.
From: Phase separation of TAZ compartmentalizes the transcription machinery to promote gene expression
a, Domain structure of TAZ and TAZ truncations. The numbers above indicate the position of amino acid residues. b, Bacterially purified GFP-TAZ, ∆TB, ∆WW, ∆CC, and ∆WW+∆CC proteins were analyzed by SDS-PAGE and detected by Coomasssie blue staining. c, Localization of GFP-TAZ and various mutants in HeLa cells. d, Localization of GFP-TAZ and various TAZ/YAP chimera in HeLa cells. Scale bars, 10 μm. e, A GST pull-down assay was performed by incubating immobilized GST fusion proteins with lysates of cells expressing HA-tagged WT or mutant TAZ, and the associated TAZ proteins were detected by western blotting with anti-HA (upper). GST fusion proteins were assessed by western blotting with anti-GST, and HA-TAZ proteins in the cell lysates were measured by western blotting (lower). Experiments in b–e were repeated independently three times with similar results. Unprocessed blots are provided in Unprocessed Blots Extended Data Fig. 3.
