Extended Data Fig. 5: Hippo signaling negatively regulates TAZ phase separation in HeLa cells.
From: Phase separation of TAZ compartmentalizes the transcription machinery to promote gene expression
TAZ localization was examined by immunofluorescence staining with anti-TAZ (green) in HeLa cells that have been subjected to the following treatments: a, Serum-starved HeLa cells were treated with 1 μM LPA or 50 ng/ml EGF for 1 h. b, Serum-starved HeLa cells were seeded on fibronectin-coated coverslips for 10 min or 2 h in serum-free medium. c, HeLa cells were grown on fibronectin-coated polyacrylamide hydrogels of 1 kPa and 40 kPa stiffness. d, HeLa cells were treated with 1 μg/ml Latrunculin B for 1 h. Alexa Fluor 555-conjugated phalloidin (Red) staining was performed to detect F-actin in b-d. Scale bar, 10 µm. Experiments in a–d were repeated independently three times with similar results.
