Extended Data Fig. 6: LATS2 regulates TAZ LLPS and recruitment of TEAD4 and BRD4.
From: Phase separation of TAZ compartmentalizes the transcription machinery to promote gene expression
a, MCF-10A cells transfected with GFP-TAZ-S89A and Flag-LATS2 were subjected to immunofluorescence staining with anti-Flag (Red). Scale bar, 10 µm. b, MCF-10A cells stably expressing siLATS1/2 were transfected with GFP-TAZ and Flag-TEAD4. TEAD localization at high cell density was detected by immunofluorescence staining with anti-Flag (Red). Scale bar, 10 μm. c, MCF-10A cells stably expressing siLATS1/2 were transfected with GFP-TAZ. Endogenous BRD4 localization was examined by immunofluorescence staining with anti-BRD4 (Red). Scale bar, 10 μm. All experiments were repeated independently three times with similar results.
