Extended Data Fig. 8: TAZ mutants lacking the CC domain still bind to LAST2 and TEAD4.
From: Phase separation of TAZ compartmentalizes the transcription machinery to promote gene expression
a, HA-tagged WT and mutant TAZ were co-transfected into 293T cells with Flag-LATS2. TAZ proteins associated with LATS2 were isolated by immunoprecipitation with anti-Flag and detected by western blotting with anti-HA antibodies (upper panels). The abundance of these proteins in the cell lysates was assessed by western blotting (lower panels). GAPDH was used as a loading control. b, Interaction of various TAZ mutants with Flag-TEAD4 was analyzed by co-IP assay as described in a. c, Interaction of various TAZ/YAP chimera with LATS2 was analyzed by co-IP as described in a. All experiments were repeated independently three times with similar results. Unprocessed blots are provided in Unprocessed Blots Extended Data Fig. 8.
