Extended Data Fig. 1: Regulation of TAZ droplet formation in vitro and nuclear puncta formation in vivo.
From: Phase separation of TAZ compartmentalizes the transcription machinery to promote gene expression
a, GFP-TAZ purified from E. coil were analysed by SDS-PAGE and visualized by Coomassie blue staining. b. 50 μM GFP-TAZ were heated-inactivated (5 min at 95 °C and immediately put on ice for 5 min) or treated with 100 μg/ml Proteinase K for 30 min at 40 °C, and then subjected to droplet formation assay in vitro in the presence of 500 mM NaCl at room temperature. c, Ectopically expressed GFP-TAZ was expressed at a lower level than endogenous TAZ in MCF-10A cells as shown by western blotting. GAPDH was used as a loading control. d, Flag-TAZ formed nuclear puncta when transfected into the MCF-10A cells, as detected by immunofluorescence staining with anti-Flag. Scale bar, 10 μm. Experiments in a–d were repeated independently three times with similar results. Unprocessed blots are provided in Unprocessed Blots Extended Data Fig. 1.
