Extended Data Fig. 3: Motif enrichment at differentially bound sites.
From: Pro-neuronal activity of Myod1 due to promiscuous binding to neuronal genes
a, Reverse search of known motifs at FLAG ChIP peaks, as defined in Fig. 2a, that are associated with significantly changing genes (fold-change ≥ 1.2, p ≤ 0.05) using HOMER33. Ascl1-enriched and common sites mainly contain bHLH motifs, while Myod1-enriched sites also contain homeodomains and other motifs. HOMER uses ZOOPS scoring (zero or one occurrence per sequence) coupled with hypergeometric enrichment calculations to determine significance. (b-c) Examples of (b) bHLH and (c) homeobox motifs that are enriched in differentially bound sites from Fig. 3.
