Extended Data Fig. 3: Role of neutrophils in inflammatory ccRCC progression.

a, b, Flow cytometric analysis of cells in the primary tumours and lungs of OS5K-3-bearing mice in Fig. 2a–c. a, Representative gating strategy used for primary tumours. The percentages of each fraction are indicated. b, Quantification of the relative neutrophil populations in primary tumours (left) and lungs (right). c–e Neutrophil reduction in 786-3K-bearing mice. Mice were inoculated with 786-3K cells and treated with an anti-Ly-6G antibody for 24 d. n = 8 (IgG) mice and n = 7 (anti-Ly-6G). c, Quantification of the relative neutrophil populations in primary tumours (left) and lungs (right). Two-sided Welch’s t test (left). d, e, Ex vivo bioluminescence imaging. In each figure, the left panels show representative images of primary (d) and metastatic lung (e) tumours. The right panels show quantification of the overall bioluminescence for the indicated groups. f–h, Neutrophil reduction in OSPa-bearing mice. Mice were inoculated with OSPa cells and treated with an anti-Ly-6G antibody for 14 d. n = 6 mice/group. f, Quantification of the relative neutrophil populations in primary tumours (left) and lungs (right). g, h, Ex vivo bioluminescence imaging. In each figure, the left panels show representative images of primary (g) and metastatic lung (h) tumours. The right panels show quantification of the overall bioluminescence for the indicated groups. i, Flow cytometric analysis of cells in the lungs of mice inoculated OS5K-3 cells from the experiments shown in Fig. 2d,e. Quantification of the relative neutrophil populations in the lungs. In quantification of the relative neutrophil populations, the number of CD11b⁺, Ly-6C^med, and SSC^high cells was compared to all living cells. In all panels in this figure, two-sided Student’s t test was performed unless otherwise described. The bars represent means ± S.D.

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