Extended Data Fig. 6: Clinical significance of SAA expression in ccRCC.
From: Epigenetic remodelling shapes inflammatory renal cancer and neutrophil-dependent metastasis
a, Re-analysis of TCGA KIRC cohort data showing expression of the indicated genes. n = 267 (stage I), n = 57 (stage II), n = 123 (stage III), and n = 83 (stage IV) patients. One-way ANOVA and Tukey’s test. b, Re-analysis of TCGA KIRC cohort data showing the relationships between the expression of each indicated gene and overall patient survival. All patient samples were divided, based on expression of the indicated genes. n =266 patients/group. Log-rank test. Hazard ratio with 95% confidence interval was also indicated. c, Immunoblotting for FLAG-tagged IκBαM and α-tubulin in IκBαM-expressing OS5K-3 cells. d, Immunoblotting for p65, α-tubulin, and HDAC1 protein expression in IκBαM-expressing OS5K-3 cells. e, Immunoblotting for LIP form of C/EBPβ and α-tubulin in LIP-expressing OS5K-3 cells. f, Reporter luciferase analysis with an SAA1 promoter–luciferase construct. The luciferase reporter with truncated SAA1 promoters introduced in OS5K-3 cells. Numbers represent the location from the TSS of SAA1. n = 4 biologically independent samples. g, Bisulphite-sequencing of the SAA1 promoter region. The black and white circles represented methylated and unmethylated CpG sites, respectively. Numbers represent the location from the TSS of SAA1. n = 17 (OSPa), n = 15 (OS5K-1 and -3), and n = 22 (OS5K-2) independent clones. h, Re-analysis of TCGA KIRC cohort data showing the methylation status near the indicated gene loci. n = 155 (stage I), n = 31 (stage II), n = 73 (stage III), and n = 58 (stage IV) patients. In all panels in this figure, the bars represent means ± S.D. One-way ANOVA and Tukey’s test.
