Extended Data Fig. 1: Malignant phenotypes of ccRCC derivatives.
From: Epigenetic remodelling shapes inflammatory renal cancer and neutrophil-dependent metastasis
a, In vivo bioluminescence imaging of mice inoculated with OS-RC-2 cells. b, Survival of OS-RC-2 derivative-bearing mice. The indicated cells were inoculated orthotopically. n = 7 (OSPa and OS5K-1) and n = 8 (OS5K-2 and -3) mice. Log-rank test. c, d, Ex vivo bioluminescence imaging of primary and metastatic lung tumours of 786-O derivatives. Mice bearing 786-O derivatives were analysed 35 d after orthotopic inoculation. In each figure, the left panels show representative images of primary tumours (c) or metastatic lung tumours (d). n = 6 (786-Pa) and n = 5 (786-3K) mice. The right panels show quantification of the overall bioluminescence for the indicated groups. e, f, Colony-formation assays for OS-RC-2 and 786-O derivatives. In each figure, the left panels show representative images of colonies from OS-RC-2 (e) and 786-O (f) derivatives. The right panels indicate the number of colonies for the indicated groups. n = 15 sights from n = 3 independent samples. g, h, Transwell assays performed with OS-RC-2 and 786-O derivatives. In each figure, the left panels show representative images of the migrated OS-RC-2 (g) and 786-O (h) derivatives. The right panels show the percentages of the areas covered by the migrated cells for the indicated groups. n = 15 sights from n = 3 independent samples. In all panels in this figure, the bars represent means ± S.D.
