Extended Data Fig. 5: Synthetic interactions between dynein mutants and Kar9.
(a–c) Serial dilutions of cells with indicated genotype were plated on rich media (YPA supplemented with 2% glucose) and grown at 30 °C for 2-4 days (a, extended incubation of plates shown in Fig. 4e were grown for 4 days; all others were incubated for 2 days). Note the severe growth defects in dyn1HL3 kar9∆ cells (in panel c), suggesting that dyneinHL3 is not active in cells. Note that similar results were obtained from 2 independent replicates.
