Extended Data Fig. 5: Multiplexed Isobaric Tag-Based Profiling of MCC-derived xenografts reveals that LSD1 inhibition perturbs neuronal gene expression in human and mouse tissues. | Nature Cell Biology

Extended Data Fig. 5: Multiplexed Isobaric Tag-Based Profiling of MCC-derived xenografts reveals that LSD1 inhibition perturbs neuronal gene expression in human and mouse tissues.

From: Merkel cell polyomavirus activates LSD1-mediated blockade of non-canonical BAF to regulate transformation and tumorigenesis

Extended Data Fig. 5

a-b, The multiplexed Isobaric Tag-Based Profiling of the MKL-1 virus-positive MCC cell line treated with GSK-LSD1 for eight days identified genes that are differentially expressed during LSD1 inhibition. n = 5 independent biological replicates were used for analysis. Selected GOTERM biological process and cellular compartment terms are shown for the upregulated (a) and downregulated (b) proteins. c, Eight mice were injected with MKL-1 or WaGa MCC cells, and when the tumor size reached 150 mm3, two of each four mice for each MCC model were treated with CPI-670242 (40 mg/kg) orally once a week for 22 days. The tumors were harvested for the TMT-10 plex isobaric tag-labeling quantitative mass spectrometry experiment. c, The heatmap shows the relative abundance of 30 most upregulated and 30 most downregulated mouse proteins after the treatment.

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