Extended Data Fig. 5: DMs may arise from rare Cx3cr1+ cells in the distal embryonic gland.
From: Tissue-resident ductal macrophages survey the mammary epithelium and facilitate tissue remodelling
a, 3D image of a Cx3cr1GFP/+ mammary rudiment at E18.5 with enlarged optical sections of the distal (i) and nipple (ii) regions, immunostained for K5 (magenta), GFP (cyan) and MHCII (yellow). Image representative of 2 mice. Arrows indicate Cx3cr1+MHCII− cells. b, 3D image of a Cx3cr1GFP/+ mammary gland at postnatal day (P) 4 with enlarged optical sections of the distal (i), mid (ii) and nipple (iii) regions, immunostained for K5 (magenta), GFP (cyan) and MHCII (yellow). Image representative of 2 mice. Arrows indicate Cx3cr1+MHCII– cells. c, 3D image of an entire Cx3cr1GFP/+ mammary gland at P7 with enlarged optical sections of the distal (i), mid (ii) and nipple (iii) regions, immunostained for K5 (magenta), GFP (cyan) and MHCII (yellow). Image representative of 2 mice. Arrows indicate dendritic Cx3cr1+MHCIIlo/+ cells. All scale bars, 300 µm (overview) and 100 µm (enlargements).
