Extended Data Fig. 7: DMs during mammary ontogeny (supporting information for Figs. 5 and 6).
From: Tissue-resident ductal macrophages survey the mammary epithelium and facilitate tissue remodelling
a, MØ FACS profiles throughout postnatal development and DM/epithelial cell (CD45–CD24+) ratio in virgin and pregnant glands. n=3, 5 weeks, preg d12.5 and preg d16.5 ; n=5, 9 weeks; n=6, lactation. Error bars, s.e.m. b, 3D images of 11 week-old adult and 16.5 d pregnant glands and enlarged optical sections, immunostained for K5 (magenta) and MHCII (yellow) and labelled for EdU (cyan). Mice were treated with EdU 2 hrs prior to collection. Images representative of 4 mice. Hollow arrow-head indicates an EdU– DM. Arrow indicates an EdU+ DM. Scale bars, 100 µm (overviews) and 15 µm (enlargements). c, 3D image and enlarged optical section of Cx3cr1GFP/+ tissue at 4 days involution, immunostained for GFP and labelled for F-actin. Images representative of 2 mice. Dotted lines indicate the outer edge of the F-actinhi basal layer. Scale bar, 30 µm. d, Optical section from a 3D image of Elf5-GFP tissue at 3 days involution. Image representative of 3 mice. Arrows indicate large GFPlo alveolar cells surrounded by MHCII signal. Hollow arrow-heads indicate binucleated cells. Scale bar, 20 µm. W, weeks; d, days; lact, lactation.
