Extended Data Fig. 7: Single Cell Transcriptome Analysis of Telogen Skin Epidermis. | Nature Cell Biology

Extended Data Fig. 7: Single Cell Transcriptome Analysis of Telogen Skin Epidermis.

From: NFI transcription factors provide chromatin access to maintain stem cell identity while preventing unintended lineage fate choices

Extended Data Fig. 7

a, FACS-purification of 2nd telogen skin progenitors from Sox9-CreER/Nfixfl/fl/R26-YFP (non-phenotypic) vs. NFI-dKO mice. Hair follicles were YFP+ and INTEGRIN α6+, while epidermal SCs (EpdSCs) were YFPneg, INTEGRIN α6+ and SCA1+. Representative plots for three biological replicates. b, Validation of FACS-purification strategy for single cell RNA-seq analysis. tSNE plot showing low Sox9 expression in the YFPneg cluster (EpdSC), whereas all YFP+ populations are Sox9+ (HF). n=2 mice per group. c, Correlation plots of single-cell RNA-seq libraries shows minimal batch to batch variation. n=2 mice per group. Correlation coefficients were calculated by Pearson’s method. d, tSNE plots showing expression of known epidermal lineage markers to determine the identity of individual clusters. n=2 mice per group. e, tSNE plot showing the unique cluster in NFI-dKO mice is Cd34neg. n=2 mice per group.

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