Extended Data Fig. 3: Control engrailed-GAL4 overexpression and junctional actomyosin quantifications.
a, Distribution of measured nuclear NLS::RFP fluo. int. and selected threshold to define the control (<475) and engrailed-GAL4 induced NLS::RFP cells (>475). b, Box plots: mean medial F-Actin fluo. int. averaged per cell and over time (90 or 120 × 10 sec), normalized to the mean of controls. c, Box plots: cell averaged S.D. of high-pass filtered total medial F-Actin (left) fluo. int. and apical cell area (right), normalized to the mean of controls. d,e, Box plots: cell averaged mean junctional MyoII (left) and F-Actin (right) intensities in ectodermal (d) or amnioserosa cells (e), normalized to the mean of controls. Quantifications and statistical analysis in a-e were carried out using n = the total number of cells gathered from multiple embryos, as indicated below graphs (e = embryos, c = cells). Box plots in b,c,d,e: as described in Fig. 1 legend. Statistics in b,c,d,e: two-sided Mann–Whitney test, NS: p > 5E-2, *: p < 5E-2, **: p < 5E-3, ***: p < 5E-4, ****: p < 5E-5. Statistical source data are provided in Source Data Extended Data Fig. 3.
