Extended Data Fig. 4: Junctional dynamics in Frl loss or gain of function during GBE.
a, Display of the shrinking and extending AJs in ectodermal cells during GBE, defined respectively as the disappearing and appearing junctions throughout movies duration. b, Line plots: averaged AJs length ± S.D. over time depicting shrinking (before reference time t = 0 min) and extending (after reference time t = 0 min) junctions. c, Line plots: one junction example of shrinking (top) and extending (bottom) AJs and the linear fit method used to extract the shrinking and extending rate. d, Box plots: junction shrinking and extending rate in WT vs frl59/59 (left) or WT vs FrlOE (right) ectodermal cells. e, Diagrams showing the reference angles used to quantify the shrinking (top) and extending (bottom) junction orientation. f, Box plots: junction orientation angle in WT vs frl59/59 (left) or WT vs FrlOE (right) ectodermal cells. Results in a-f are representative of 5 (2 control, 3 frl59/59) and 5 (3 control, 2 FrlOE) independent experiments. Quantifications and statistical analysis in b,d,f were carried out using n = the total number of junctions gathered from multiple embryos, as indicated below graphs (e = embryos, j = junctions). Box plots in d,f: as described in Fig. 1 legend. Statistics in d,f: two-sided Mann–Whitney test, NS: p > 5E-2, *: p < 5E-2, **: p < 5E-3, ***: p < 5E-4, ****: p < 5E-5. Statistical source data are provided in Source Data Extended Data Fig. 4.
