Extended Data Fig. 4: Attenuated crypt regeneration capacity in Irf2–/–; Ifnar1–/– and Irf2–/–; Ifnar1–/–; Stat1–/– mice, and the expression of IFN-inducible genes in the Irf2–/–; Ifnar1–/–; Stat1–/– mice.
a, Jejunum sections from 5-FU-treated Irf2+/–;Ifnar1–/– and Irf2–/–;Ifnar1–/– mice were stained with Ki67. Image representative of five mice per group. Scale bars: 500 µm. b, Viable regenerating crypts per intestinal circumference were counted in the Ki67-stained jejunum from Irf2+/−;Ifnar1–/– (n = 6 mice, average number of viable crypts of 5–7 sections per mouse were counted) and from Irf2−/−;Ifnar1–/– (n = 5 mice, average number of viable crypts of 5–7 sections per mouse were counted) mice. Data represents means ± s.d. c, Jejunum sections from 5-FU-treated Irf2+/−;Ifnar1–/–;Stat1–/– and Irf2−/−;Ifnar1–/–;Stat1–/– mice were stained with Ki67. Image representative of five mice per group. Scale bars: 500 µm. d, Viable regenerating crypts per intestinal circumference were counted in the Ki67-stained jejunum from Irf2+/−;Ifnar1–/–;Stat1–/– (n = 5 mice, average number of viable crypts of 5–7 sections per mouse were counted) and from Irf2−/−;Ifnar1–/–;Stat1–/– (n = 5 mice, average number of viable crypts of 5–7 sections per mouse were counted) mice. Data represent means ± s.d. e-g, The expression of Ifit1 (e), Ifit2 (f) and Tapbp (g) in isolated crypt epithelial cells of Irf2+/– and of Irf2–/– mice (n = 5 or 7 mice per group). h-j, The expression of Ifit1 (h), Ifit2 (i), Tapbp (j) in FCM sorted crypt ECs of Irf2+/–;Ifnar1–/–;Stat1–/– mice and of Irf2–/–;Ifnar1–/–;Stat1–/– mice (n = 5 or 7 mice per group). Data represent means of relative expression ± s.d. Statistics were determined by two-tailed Mann-Whitney test.
