Extended Data Fig. 7: Phenotypic and functional characterization of the transcriptome after KD of SOX10.
From: Robust gene expression programs underlie recurrent cell states and phenotype switching in melanoma
a, Experimental setup of scRNA-seq after KD of SOX10. b, Western blot for SOX10, and GAPDH as control, after KD of SOX10 (the same time points) in the same three MM lines (MM074, MM087 and MM057; WB performed once). c, Pie charts showing fractions of cells in different cell cycle phases for MM074, MM087 and MM057 at baseline and control KD conditions together with different time points after KD of SOX10. d, Comparative alignment of transition trajectories using different methods. Trajectories predicted by DiffusionMap, Scorpius and Monocle-2 were aligned by applying dynamic time warping, using cellAlign, and the predicted optimal alignment is shown with a white line. The concordance between different methods is high.
