Extended Data Fig. 5: Pir1 prevents untimely gene expression in suboptimal conditions.
a, Genes within the ‘meiotic coding’ and ‘non-meiotic coding’ groups in Fig. 4c were categorized separately based on GO biological process. b, Growth curves of WT and pir1∆ cells cultured in rich or minimal medium at 26 °C. Optical density (OD) at 595 nm wavelength is plotted. Data representative of two independent experiments are shown. c, Percent cell death was determined by methylene blue staining of cells grown on minimal medium at 26 °C. Two independent experiments were performed with similar results. d, Heatmap of log2 fold changes in expression in WT and pir1∆ cells grown in minimal or rich medium relative to WT cells grown in rich medium at 30 °C. Transcripts were clustered based on their function. ‘n’ indicates number of loci in each category. Data representative of two independent experiments are presented. e, Schematic depicting how fluctuations in gametogenic gene expression, which occur upon changes in growth conditions, are controlled by MTREC–Pir1. f, Serial dilutions of the indicated strains were spotted onto rich medium and incubated at the indicated temperature for 3-4 days. Two independent experiments were performed with similar results. Source data are provided.
