Extended Data Fig. 6: Synchronized meiosis system.
a, Flowchart of pat1-as2 induced synchronized meiosis. b, Progression of meiosis was monitored by fluorescence microscopy to determine the number of nuclei per cell in samples collected at the indicated time points after the addition of 1-NM-PP1. Two independent experiments were performed with similar results. c, RT–qPCR analysis of meiotic gene expression. Samples were collected at the indicated time points. Two independent experiments were performed with similar results. d, Heatmap of log2 fold changes in expression relative to vegetative cells for synchronized meiotic cells, as determined by RNA-seq analyses. Clusters are grouped by expression pattern and only transcripts with peak log2 fold changes that were ≥1.5 were assigned to a group. Data representative of two independent experiments are shown. Source data are provided.
