Extended Data Fig. 4: DACT1 bodies are proteasomally degraded and do not co-localize with known organelles.
From: TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis
a, DACT1-TdTomato fusion protein expression in M1a cells with or without 24 h Wnt3a treatment. Scale bar represents 10 μm. Representative of n > 5 independent replicates. b, DACT1-TdTomato c-terminal fusions imaged in HPL1 cells. Scale bar represents 5 μm. Representative of n > 5 independent replicates. c, d, Western blot analysis of DACT1 wild-type and DACT1-TdTomato fusion protein expression in both M1a and BM2 cells after treatment with cycloheximide (50 μg/mL) at the indicated times (c) or either Bafilomycin A (10 nM) or MG-132 (20 μM) for 12 h. (d). Images (c, d) representative of 3 independent experiments. * indicates expected molecular weight of fusion proteins. e, Representative images of DACT1-TdTomato fusions and the indicated organelle-associated proteins imaged by confocal sectioning. Scale bars represent 4 μm. Representative of 3 independent replicates. Uncropped blots for c, d, are provided.
