Extended Data Fig. 6: Deletion and hybrid mutant analysis of DACT1 protein localization.
From: TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis
a, Schematic of deletions made in the DACT1 amino acid sequence with predicted intrinsically disordered domains in black background and previously reported sequence motifs labeled in multiple colors. b, c, Confocal imaging of BM2 (c) and M1a (d) cells expressing DR7-TdTomato or DR8-TdTomato fusions labeled with Hoechst. Scale bars represent 10 μm. Representative of >3 independent replicates. d, Fluorescent in situ hybridization of RNA probes for Ctnnb1 in BM2-hDACT1-TdTomato expressing cells. Scale bars represent 20 μm. Representative of 3 independent replicates.
