Extended Data Fig. 2: DACT1 represses Wnt signaling.
From: TGF-β-induced DACT1 biomolecular condensates repress Wnt signalling to promote bone metastasis
a,b, qPCR analysis of DACT1 mRNA levels normalized to Gapdh in BM2-TGC (a) and HPL1-TGC (b) cells stably transduced with DACT1-targeting shRNA (KD#1 and KD#2) or overexpression constructs. n = 4 technical repeats, Student’s t-test. Data represents mean ± SEM. Student’s t-test. c, d, Flow cytometry assessment of 7x-TCF-GFP Wnt reporter activity in BM2 (c) or HPL1 (d) cells with DACT1 knockdown or overexpression, n = 3 (c) and n = 4 (d) biological replicates. Student’s t-test. Experiments were independently repeated >3 times (c) and 2 times (d). e, Immunofluorescence of total β-catenin in BM2 cells with DACT1 knockdown or overexpression with Wnt3a stimulation for 24 hours. Scale bars represent 50 μm. Representative of 2 independent replicates. f, Western blot of indicated proteins in BM2 cells with indicated DACT1-related constructs with Wnt3a or control media stimulation for 24 hours. Representative of 2 independent replicates. Numerical source data for a-d and uncropped blots for f, are provided.
