Extended Data Fig. 1: Fcho2 assembles into protein-rich domains with Eps15 on membranes and in solution.
From: Liquid-like protein interactions catalyse assembly of endocytic vesicles
a-e, Fcho2 is labeled with Atto-594. Eps15 contains an N-terminal 6xHis tag and is labeled with CF488a. a-b, Center slices (upper panels) and corresponding z-projections (lower panels) of representative GUVs incubated with 500 nM of the indicated protein(s). GUVs contain 79% DOPC, 15% DOPS, 5% PtdIns(4,5)P2, and 1% DPEG10-biotin. a, Full-length Fcho2 alone decorates GUVs homogeneously. b, GUVs incubated with both Fcho2 and Eps15 display protein-rich domains. c, 7 μM Fcho2 clusters into small, irregular aggregates. d, When combined at a 1:34 ratio, Fcho2 and Eps15 colocalize in protein droplets. e, Time course of a fusion event between droplets containing Fcho2 (magenta) and Eps15 (green). Scale bars are 5 μm in a-c, e and 10 µm in d.
