Extended Data Fig. 3: Eps15/Fcho1 droplet:solution partitioning decreases at increasing temperature.
From: Liquid-like protein interactions catalyse assembly of endocytic vesicles
a-c, Representative images of protein droplets at increasing temperatures, followed by the return to room temperature after heat removal. Plots show fluorescence intensity of Eps15-CF488a measured along dotted lines in each image. Intensity is normalized to the maximum value in the first 25 °C panel for each set of images. Fcho1 is unlabeled. Total protein concentration is held at 7 μM while Fcho1:Eps15 ratio is varied. Droplets are formed from (a) 7 μM Eps15, (b) 0.1 μM Fcho1, 6.9 μM Eps15, and (c) 0.2 μM Fcho1, 6.8 μM Eps15. See Source Data Extended Data Fig. 3.
