Extended Data Fig. 6: Accumulation of single-stranded DNA upon MSL disruption.
From: Disruption of the MSL complex inhibits tumour maintenance by exacerbating chromosomal instability
a-g, Immunofluorescence microscopy of MSLLOF TDF cells using the indicated antibodies (a-d, f-g) and quantification of DAPI intensity in nuclei and micronuclei (e). Nuclei were counterstained with DAPI. Yellow arrowheads in g indicate mitotic cells displaying p-RPA foci on chromosomes. N = 172 biologically independent cells collected from two experiments. P-value from one tailed paired Student’s t-test. Scale bar: 10 µm. h, Aphidicolin sensitivity assay comparing TDF and normal HME1 cells. Values are average ± SEM from n = 3 biologically independent samples after 5 days of growth. The non-linear fit of the experimental points is shown. Minor differences in TDF IC50 compared to Fig. 4g are due to a slightly different set up of the experiment (see Methods). i, Clonogenic assays comparing the proliferative capacity of individual TDF cells of the indicated genotypes (See also Fig. 6a). N = 103, 131, 161 biologically independent clones for WT, cntr-KO and MSLLOF(MSL3-KO), respectively, collected from two experiments. Horizontal lines: median values. P-value from one-way ANOVA followed by Dunn’s test. N.s.: non-significant (p-value = 0.9495). Source data are provided.
