Extended Data Fig. 1: Characterization of endothelial struts.
From: Endothelial struts enable the generation of large lumenized blood vessels de novo
a, All ECs are marked by both laCherry (magenta; cytoskeleton) and eGFP (green; nuclei). Struts are depicted by arrowheads. Images are representative of 20 embryos analysed from two independent experiments. b, Quantification of the number of endothelial struts at 22 hpf and 26 hpf by confocal microscopy and represented as the median values with the first and third quartiles; the whiskers represent the minimum and maximum (4 different clutches per time point (n = 26; n = 25; n = 27; n = 25 for 22 hpf and n = 25; n = 28; n = 25; n = 25 for 26 hpf. Two-tailed Student’s t-test. ***P < 0.0001 for all 4 tests). Arrowheads depict endothelial struts. Images are representative of indicated groups from four independent experiments. c, Strut formation in the anterior part of the PCV at 22hpf by confocal microscopy and represented as the median values with the first and third quartiles; the whiskers represent the minimum and maximum (n = 30 embryos from three independent experiments). Arrowheads depict endothelial struts. d, Quantification of the number of acridine orange positive ECs in the CV region at 20 hpf and representative confocal images. White arrowhead depicts a positive EC (n = 20 embryos from two independent experiments). Scale bars are defined in the figure.
